######################## # Kinetic parameters # ######################## ############################# # Hydrogen exchange rates # ############################# save_H_exch_rate_list_1 _H_exch_rate_list.Sf_category H_exch_rates _H_exch_rate_list.Sf_framecode H_exch_rate_list_1 _H_exch_rate_list.Entry_ID 17018 _H_exch_rate_list.ID 1 _H_exch_rate_list.Sample_condition_list_ID 1 _H_exch_rate_list.Sample_condition_list_label $sample_conditions_1 _H_exch_rate_list.Val_units s _H_exch_rate_list.Details ; After recording reference 15N-HSQC spectra, 15N-labeled DAXX55-144 in 500 microL protonated NMR buffer was lyophilized and re-dissolved in an equivalent volume of D2O. Amide exchange rates were measured by recording a series of sensitivity-enhanced 15N-HSQC spectra at 298K, starting ~5 min after the addition of D2O. ; _H_exch_rate_list.Text_data_format . _H_exch_rate_list.Text_data . loop_ _H_exch_rate_experiment.Experiment_ID _H_exch_rate_experiment.Experiment_name _H_exch_rate_experiment.Sample_ID _H_exch_rate_experiment.Sample_label _H_exch_rate_experiment.Sample_state _H_exch_rate_experiment.Entry_ID _H_exch_rate_experiment.H_exch_rate_list_ID 1 '2D 1H-15N HSQC' . . . 17018 1 stop_ loop_ _H_exch_rate.ID _H_exch_rate.Assembly_atom_ID _H_exch_rate.Entity_assembly_ID _H_exch_rate.Entity_ID _H_exch_rate.Comp_index_ID _H_exch_rate.Seq_ID _H_exch_rate.Comp_ID _H_exch_rate.Atom_ID _H_exch_rate.Atom_type _H_exch_rate.Atom_isotope_number _H_exch_rate.Val _H_exch_rate.Val_min _H_exch_rate.Val_max _H_exch_rate.Val_err _H_exch_rate.Resonance_ID _H_exch_rate.Auth_entity_assembly_ID _H_exch_rate.Auth_seq_ID _H_exch_rate.Auth_comp_ID _H_exch_rate.Auth_atom_ID _H_exch_rate.Entry_ID _H_exch_rate.H_exch_rate_list_ID 1 . 1 1 15 15 LYS H H 1 736.2 . . . . 1 65 LYS H 17018 1 2 . 1 1 16 16 LEU H H 1 7644 . . . . 1 66 LEU H 17018 1 3 . 1 1 17 17 PHE H H 1 18760 . . . . 1 67 PHE H 17018 1 4 . 1 1 18 18 GLU H H 1 55040 . . . . 1 68 GLU H 17018 1 5 . 1 1 19 19 GLU H H 1 45590 . . . . 1 69 GLU H 17018 1 6 . 1 1 20 20 PHE H H 1 112800 . . . . 1 70 PHE H 17018 1 7 . 1 1 21 21 LEU H H 1 161900 . . . . 1 71 LEU H 17018 1 8 . 1 1 22 22 GLU H H 1 106700 . . . . 1 72 GLU H 17018 1 9 . 1 1 23 23 LEU H H 1 148600 . . . . 1 73 LEU H 17018 1 10 . 1 1 24 24 CYS H H 1 22810 . . . . 1 74 CYS H 17018 1 11 . 1 1 50 50 LEU H H 1 1634 . . . . 1 100 LEU H 17018 1 12 . 1 1 55 55 PHE H H 1 2021 . . . . 1 105 PHE H 17018 1 13 . 1 1 56 56 CYS H H 1 5686 . . . . 1 106 CYS H 17018 1 14 . 1 1 58 58 ILE H H 1 5027 . . . . 1 108 ILE H 17018 1 15 . 1 1 59 59 LEU H H 1 124900 . . . . 1 109 LEU H 17018 1 16 . 1 1 60 60 SER H H 1 5887 . . . . 1 110 SER H 17018 1 17 . 1 1 61 61 ARG H H 1 2228 . . . . 1 111 ARG H 17018 1 18 . 1 1 63 63 LEU H H 1 44280 . . . . 1 113 LEU H 17018 1 19 . 1 1 64 64 SER H H 1 623.2 . . . . 1 114 SER H 17018 1 20 . 1 1 76 76 TYR H H 1 10390 . . . . 1 126 TYR H 17018 1 21 . 1 1 77 77 ILE H H 1 23820 . . . . 1 127 ILE H 17018 1 22 . 1 1 80 80 LEU H H 1 25340 . . . . 1 130 LEU H 17018 1 23 . 1 1 81 81 CYS H H 1 13310 . . . . 1 131 CYS H 17018 1 24 . 1 1 83 83 VAL H H 1 1901 . . . . 1 133 VAL H 17018 1 25 . 1 1 85 85 LYS H H 1 2353 . . . . 1 135 LYS H 17018 1 stop_ save_