######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronuclear_T1_list_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_1 _Heteronucl_T1_list.Entry_ID 26519 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 500 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ground State Values of obtained from fits of all CEST profiles recorded on the Abp1p-Ark1p system, 1C, 11.7T to a global two-site exchange processes. Values of (kex,pE) were fixed to (130 s-1, 2.38%) and profiles for all of the residues fit simultaneously. The following six residues showed clear evidence for a second state (well resolved dips for both ground and excited states in profiles): 15 31 32 33 34 37 ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 '2D 1H-15N HSQC 1' . . . 26519 1 2 '2D 1H-15N HSQC 2' . . . 26519 1 3 '2D 1H-15N HMQC 1' . . . 26519 1 4 '2D 1H-15N HMQC 2' . . . 26519 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 1 1 ALA N N 15 1.60 0.01 . . . . . 26519 1 2 . 1 1 3 3 TRP N N 15 1.87 0.01 . . . . . 26519 1 3 . 1 1 4 4 ALA N N 15 2.02 0.01 . . . . . 26519 1 4 . 1 1 5 5 THR N N 15 1.88 0.01 . . . . . 26519 1 5 . 1 1 6 6 ALA N N 15 1.97 0.01 . . . . . 26519 1 6 . 1 1 7 7 GLU N N 15 1.98 0.02 . . . . . 26519 1 7 . 1 1 8 8 TYR N N 15 1.92 0.01 . . . . . 26519 1 8 . 1 1 9 9 ASP N N 15 1.66 0.01 . . . . . 26519 1 9 . 1 1 10 10 TYR N N 15 1.96 0.01 . . . . . 26519 1 10 . 1 1 11 11 ASP N N 15 1.75 0.01 . . . . . 26519 1 11 . 1 1 12 12 ALA N N 15 1.81 0.01 . . . . . 26519 1 12 . 1 1 13 13 ALA N N 15 1.81 0.01 . . . . . 26519 1 13 . 1 1 14 14 GLU N N 15 1.64 0.01 . . . . . 26519 1 14 . 1 1 15 15 ASP N N 15 1.67 0.01 . . . . . 26519 1 15 . 1 1 16 16 ASN N N 15 1.79 0.01 . . . . . 26519 1 16 . 1 1 17 17 GLU N N 15 1.86 0.01 . . . . . 26519 1 17 . 1 1 18 18 LEU N N 15 2.05 0.01 . . . . . 26519 1 18 . 1 1 19 19 THR N N 15 1.67 0.01 . . . . . 26519 1 19 . 1 1 20 20 PHE N N 15 1.98 0.01 . . . . . 26519 1 20 . 1 1 21 21 VAL N N 15 1.86 0.01 . . . . . 26519 1 21 . 1 1 22 22 GLU N N 15 1.78 0.01 . . . . . 26519 1 22 . 1 1 23 23 ASN N N 15 1.91 0.01 . . . . . 26519 1 23 . 1 1 24 24 ASP N N 15 1.89 0.01 . . . . . 26519 1 24 . 1 1 25 25 LYS N N 15 1.77 0.01 . . . . . 26519 1 25 . 1 1 27 27 ILE N N 15 1.99 0.01 . . . . . 26519 1 26 . 1 1 28 28 ASN N N 15 1.81 0.01 . . . . . 26519 1 27 . 1 1 29 29 ILE N N 15 1.98 0.03 . . . . . 26519 1 28 . 1 1 30 30 GLU N N 15 1.87 0.01 . . . . . 26519 1 29 . 1 1 31 31 PHE N N 15 1.64 0.01 . . . . . 26519 1 30 . 1 1 32 32 VAL N N 15 1.70 0.01 . . . . . 26519 1 31 . 1 1 33 33 ASP N N 15 1.97 0.01 . . . . . 26519 1 32 . 1 1 34 34 ASP N N 15 1.70 0.01 . . . . . 26519 1 33 . 1 1 35 35 ASP N N 15 1.84 0.01 . . . . . 26519 1 34 . 1 1 36 36 TRP N N 15 1.83 0.01 . . . . . 26519 1 35 . 1 1 37 37 TRP N N 15 1.96 0.01 . . . . . 26519 1 36 . 1 1 38 38 LEU N N 15 1.90 0.01 . . . . . 26519 1 37 . 1 1 39 39 GLY N N 15 1.93 0.01 . . . . . 26519 1 38 . 1 1 40 40 GLU N N 15 1.48 0.01 . . . . . 26519 1 39 . 1 1 41 41 LEU N N 15 2.00 0.01 . . . . . 26519 1 40 . 1 1 42 42 GLU N N 15 1.96 0.01 . . . . . 26519 1 41 . 1 1 43 43 LYS N N 15 1.89 0.01 . . . . . 26519 1 42 . 1 1 44 44 ASP N N 15 1.96 0.01 . . . . . 26519 1 43 . 1 1 45 45 GLY N N 15 1.92 0.01 . . . . . 26519 1 44 . 1 1 46 46 SER N N 15 1.91 0.01 . . . . . 26519 1 45 . 1 1 47 47 LYS N N 15 1.70 0.01 . . . . . 26519 1 46 . 1 1 48 48 GLY N N 15 1.91 0.01 . . . . . 26519 1 47 . 1 1 49 49 LEU N N 15 1.84 0.01 . . . . . 26519 1 48 . 1 1 50 50 PHE N N 15 2.04 0.01 . . . . . 26519 1 49 . 1 1 52 52 SER N N 15 1.88 0.01 . . . . . 26519 1 50 . 1 1 53 53 ASN N N 15 1.85 0.01 . . . . . 26519 1 51 . 1 1 54 54 TYR N N 15 1.99 0.01 . . . . . 26519 1 52 . 1 1 55 55 VAL N N 15 1.95 0.01 . . . . . 26519 1 53 . 1 1 56 56 SER N N 15 1.87 0.01 . . . . . 26519 1 54 . 1 1 57 57 LEU N N 15 1.76 0.01 . . . . . 26519 1 55 . 1 1 58 58 GLY N N 15 1.91 0.01 . . . . . 26519 1 56 . 1 1 59 59 ASN N N 15 1.71 0.01 . . . . . 26519 1 stop_ save_