######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronuclear_T2_list_G_State _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_G_State _Heteronucl_T2_list.Entry_ID 26519 _Heteronucl_T2_list.ID 1 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 500 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ground State Values of obtained from fits of all CEST profiles recorded on the Abp1p-Ark1p system, 1C, 11.7T to a global two-site exchange processes. Values of (kex,pE) were fixed to (130 s-1, 2.38%) and profiles for all of the residues fit simultaneously. The following six residues showed clear evidence for a second state (well resolved dips for both ground and excited states in profiles): 15 31 32 33 34 37 ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 1 '2D 1H-15N HSQC 1' . . . 26519 1 2 '2D 1H-15N HSQC 2' . . . 26519 1 3 '2D 1H-15N HMQC 1' . . . 26519 1 4 '2D 1H-15N HMQC 2' . . . 26519 1 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 1 1 ALA N N 15 5.33 0.25 . . . . . . . 26519 1 2 . 1 1 3 3 TRP N N 15 8.71 0.36 . . . . . . . 26519 1 3 . 1 1 4 4 ALA N N 15 8.78 0.39 . . . . . . . 26519 1 4 . 1 1 5 5 THR N N 15 9.63 0.30 . . . . . . . 26519 1 5 . 1 1 6 6 ALA N N 15 7.09 0.45 . . . . . . . 26519 1 6 . 1 1 7 7 GLU N N 15 9.28 1.39 . . . . . . . 26519 1 7 . 1 1 8 8 TYR N N 15 8.87 0.25 . . . . . . . 26519 1 8 . 1 1 9 9 ASP N N 15 8.48 0.23 . . . . . . . 26519 1 9 . 1 1 10 10 TYR N N 15 9.57 0.24 . . . . . . . 26519 1 10 . 1 1 11 11 ASP N N 15 8.52 0.21 . . . . . . . 26519 1 11 . 1 1 12 12 ALA N N 15 8.46 0.32 . . . . . . . 26519 1 12 . 1 1 13 13 ALA N N 15 8.72 0.25 . . . . . . . 26519 1 13 . 1 1 14 14 GLU N N 15 8.05 0.22 . . . . . . . 26519 1 14 . 1 1 15 15 ASP N N 15 8.27 0.10 . . . . . . . 26519 1 15 . 1 1 16 16 ASN N N 15 8.18 0.19 . . . . . . . 26519 1 16 . 1 1 17 17 GLU N N 15 9.64 0.14 . . . . . . . 26519 1 17 . 1 1 18 18 LEU N N 15 9.49 0.34 . . . . . . . 26519 1 18 . 1 1 19 19 THR N N 15 8.16 0.19 . . . . . . . 26519 1 19 . 1 1 20 20 PHE N N 15 9.19 0.65 . . . . . . . 26519 1 20 . 1 1 21 21 VAL N N 15 9.45 0.60 . . . . . . . 26519 1 21 . 1 1 22 22 GLU N N 15 7.92 0.39 . . . . . . . 26519 1 22 . 1 1 23 23 ASN N N 15 8.36 0.35 . . . . . . . 26519 1 23 . 1 1 24 24 ASP N N 15 9.65 0.38 . . . . . . . 26519 1 24 . 1 1 25 25 LYS N N 15 8.53 0.37 . . . . . . . 26519 1 25 . 1 1 27 27 ILE N N 15 8.39 0.77 . . . . . . . 26519 1 26 . 1 1 28 28 ASN N N 15 8.46 0.56 . . . . . . . 26519 1 27 . 1 1 29 29 ILE N N 15 9.12 2.46 . . . . . . . 26519 1 28 . 1 1 30 30 GLU N N 15 9.35 0.23 . . . . . . . 26519 1 29 . 1 1 31 31 PHE N N 15 9.73 0.13 . . . . . . . 26519 1 30 . 1 1 32 32 VAL N N 15 7.71 0.13 . . . . . . . 26519 1 31 . 1 1 33 33 ASP N N 15 8.12 0.18 . . . . . . . 26519 1 32 . 1 1 34 34 ASP N N 15 8.07 0.11 . . . . . . . 26519 1 33 . 1 1 35 35 ASP N N 15 8.36 0.16 . . . . . . . 26519 1 34 . 1 1 36 36 TRP N N 15 8.47 0.23 . . . . . . . 26519 1 35 . 1 1 37 37 TRP N N 15 9.91 0.28 . . . . . . . 26519 1 36 . 1 1 38 38 LEU N N 15 9.29 0.51 . . . . . . . 26519 1 37 . 1 1 39 39 GLY N N 15 9.60 0.34 . . . . . . . 26519 1 38 . 1 1 40 40 GLU N N 15 2.94 0.14 . . . . . . . 26519 1 39 . 1 1 41 41 LEU N N 15 9.51 0.33 . . . . . . . 26519 1 40 . 1 1 42 42 GLU N N 15 8.55 0.25 . . . . . . . 26519 1 41 . 1 1 43 43 LYS N N 15 8.43 0.71 . . . . . . . 26519 1 42 . 1 1 44 44 ASP N N 15 8.49 0.29 . . . . . . . 26519 1 43 . 1 1 45 45 GLY N N 15 8.98 0.46 . . . . . . . 26519 1 44 . 1 1 46 46 SER N N 15 8.36 0.44 . . . . . . . 26519 1 45 . 1 1 47 47 LYS N N 15 7.20 0.37 . . . . . . . 26519 1 46 . 1 1 48 48 GLY N N 15 8.93 0.68 . . . . . . . 26519 1 47 . 1 1 49 49 LEU N N 15 9.55 0.22 . . . . . . . 26519 1 48 . 1 1 50 50 PHE N N 15 9.78 0.69 . . . . . . . 26519 1 49 . 1 1 52 52 SER N N 15 12.14 0.23 . . . . . . . 26519 1 50 . 1 1 53 53 ASN N N 15 12.74 0.33 . . . . . . . 26519 1 51 . 1 1 54 54 TYR N N 15 9.45 0.64 . . . . . . . 26519 1 52 . 1 1 55 55 VAL N N 15 9.97 0.32 . . . . . . . 26519 1 53 . 1 1 56 56 SER N N 15 9.46 0.42 . . . . . . . 26519 1 54 . 1 1 57 57 LEU N N 15 9.26 0.38 . . . . . . . 26519 1 55 . 1 1 58 58 GLY N N 15 8.89 0.46 . . . . . . . 26519 1 56 . 1 1 59 59 ASN N N 15 6.60 0.37 . . . . . . . 26519 1 stop_ save_ ######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronuclear_T2_list_E_State _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_E_State _Heteronucl_T2_list.Entry_ID 26519 _Heteronucl_T2_list.ID 2 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 500 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Excited State Values of obtained from fits of all CEST profiles recorded on the Abp1p-Ark1p system, 1C, 11.7T to a global two-site exchange processes. Values of (kex,pE) were fixed to (130 s-1, 2.38%) and profiles for all of the residues fit simultaneously. The following six residues showed clear evidence for a second state (well resolved dips for both ground and excited states in profiles): 15 31 32 33 34 37 ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 1 '2D 1H-15N HSQC 1' . . . 26519 2 2 '2D 1H-15N HSQC 2' . . . 26519 2 3 '2D 1H-15N HMQC 1' . . . 26519 2 4 '2D 1H-15N HMQC 2' . . . 26519 2 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 1 1 ALA N N 15 0.7 10.2 . . . . . . . 26519 2 2 . 1 1 3 3 TRP N N 15 4.8 14.5 . . . . . . . 26519 2 3 . 1 1 4 4 ALA N N 15 2.6 15.5 . . . . . . . 26519 2 4 . 1 1 5 5 THR N N 15 16.4 11.8 . . . . . . . 26519 2 5 . 1 1 6 6 ALA N N 15 94.7 28.5 . . . . . . . 26519 2 6 . 1 1 7 7 GLU N N 15 12.3 60.4 . . . . . . . 26519 2 7 . 1 1 8 8 TYR N N 15 5.7 8.5 . . . . . . . 26519 2 8 . 1 1 9 9 ASP N N 15 0.1 8.8 . . . . . . . 26519 2 9 . 1 1 10 10 TYR N N 15 19.4 9.5 . . . . . . . 26519 2 10 . 1 1 11 11 ASP N N 15 3.8 8.7 . . . . . . . 26519 2 11 . 1 1 12 12 ALA N N 15 2.4 13.4 . . . . . . . 26519 2 12 . 1 1 13 13 ALA N N 15 10.9 9.8 . . . . . . . 26519 2 13 . 1 1 14 14 GLU N N 15 3.2 8.1 . . . . . . . 26519 2 14 . 1 1 15 15 ASP N N 15 13.4 3.6 . . . . . . . 26519 2 15 . 1 1 16 16 ASN N N 15 18.6 7.7 . . . . . . . 26519 2 16 . 1 1 17 17 GLU N N 15 7.1 4.5 . . . . . . . 26519 2 17 . 1 1 18 18 LEU N N 15 1.3 12.2 . . . . . . . 26519 2 18 . 1 1 19 19 THR N N 15 4.8 7.6 . . . . . . . 26519 2 19 . 1 1 20 20 PHE N N 15 17.1 28.3 . . . . . . . 26519 2 20 . 1 1 21 21 VAL N N 15 2.9 24.9 . . . . . . . 26519 2 21 . 1 1 22 22 GLU N N 15 58.4 21.5 . . . . . . . 26519 2 22 . 1 1 23 23 ASN N N 15 15.2 15.7 . . . . . . . 26519 2 23 . 1 1 24 24 ASP N N 15 7.4 16.3 . . . . . . . 26519 2 24 . 1 1 25 25 LYS N N 15 2.6 14.8 . . . . . . . 26519 2 25 . 1 1 27 27 ILE N N 15 39.7 38.3 . . . . . . . 26519 2 26 . 1 1 28 28 ASN N N 15 47.8 28.9 . . . . . . . 26519 2 27 . 1 1 29 29 ILE N N 15 22.7 110 . . . . . . . 26519 2 28 . 1 1 30 30 GLU N N 15 3.0 7.7 . . . . . . . 26519 2 29 . 1 1 31 31 PHE N N 15 6.3 4.2 . . . . . . . 26519 2 30 . 1 1 32 32 VAL N N 15 19.9 5.2 . . . . . . . 26519 2 31 . 1 1 33 33 ASP N N 15 13.1 6.9 . . . . . . . 26519 2 32 . 1 1 34 34 ASP N N 15 27.7 4.4 . . . . . . . 26519 2 33 . 1 1 35 35 ASP N N 15 22.2 6.0 . . . . . . . 26519 2 34 . 1 1 36 36 TRP N N 15 12.8 8.0 . . . . . . . 26519 2 35 . 1 1 37 37 TRP N N 15 4.0 8.8 . . . . . . . 26519 2 36 . 1 1 38 38 LEU N N 15 2.5 19.4 . . . . . . . 26519 2 37 . 1 1 39 39 GLY N N 15 18.1 13.8 . . . . . . . 26519 2 38 . 1 1 40 40 GLU N N 15 0.8 5.5 . . . . . . . 26519 2 39 . 1 1 41 41 LEU N N 15 2.4 13.3 . . . . . . . 26519 2 40 . 1 1 42 42 GLU N N 15 0.2 10.1 . . . . . . . 26519 2 41 . 1 1 43 43 LYS N N 15 18.4 33.0 . . . . . . . 26519 2 42 . 1 1 44 44 ASP N N 15 1.5 11.2 . . . . . . . 26519 2 43 . 1 1 45 45 GLY N N 15 0.6 18.4 . . . . . . . 26519 2 44 . 1 1 46 46 SER N N 15 25.1 20.2 . . . . . . . 26519 2 45 . 1 1 47 47 LYS N N 15 66.9 20.6 . . . . . . . 26519 2 46 . 1 1 48 48 GLY N N 15 23.8 31.6 . . . . . . . 26519 2 47 . 1 1 49 49 LEU N N 15 15.9 8.5 . . . . . . . 26519 2 48 . 1 1 50 50 PHE N N 15 1.2 27.9 . . . . . . . 26519 2 49 . 1 1 52 52 SER N N 15 44.7 9.1 . . . . . . . 26519 2 50 . 1 1 53 53 ASN N N 15 0.4 10.1 . . . . . . . 26519 2 51 . 1 1 54 54 TYR N N 15 5.9 26.8 . . . . . . . 26519 2 52 . 1 1 55 55 VAL N N 15 176.8 26.0 . . . . . . . 26519 2 53 . 1 1 56 56 SER N N 15 35.4 20.0 . . . . . . . 26519 2 54 . 1 1 57 57 LEU N N 15 39.7 19.0 . . . . . . . 26519 2 55 . 1 1 58 58 GLY N N 15 2.2 18.0 . . . . . . . 26519 2 56 . 1 1 59 59 ASN N N 15 0.5 14.9 . . . . . . . 26519 2 stop_ save_