######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronuclear_T1_list_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_1 _Heteronucl_T1_list.Entry_ID 26520 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 500 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ground State Values of obtained from fits of all CEST profiles recorded on the A39G FF domain, 1C, 11.7T to a global two-site exchange processes. Values of (kex,pE) were fixed to (51.6 s-1,1.65%) and profiles for all of the residues fit simultaneously. The 37 residues that showed clear evidence for a second state are as follows (well resolved dips for both ground and excited states in profiles) 10 13 15 18 22 25 26 27 28 29 33 37 38 39 41 42 43 44 45 48 50 51 52 53 54 55 56 59 60 61 63 64 65 66 67 68 69 ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 '2D 1H-15N HSQC' . . . 26520 1 2 '2D 1H-15N HMQC' . . . 26520 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 3 3 GLN N N 15 1.19 0.01 . . . . . 26520 1 2 . 1 1 5 5 ALA N N 15 1.43 0.01 . . . . . 26520 1 3 . 1 1 6 6 LYS N N 15 1.49 0.01 . . . . . 26520 1 4 . 1 1 7 7 LYS N N 15 1.56 0.01 . . . . . 26520 1 5 . 1 1 8 8 THR N N 15 1.54 0.01 . . . . . 26520 1 6 . 1 1 9 9 TYR N N 15 1.53 0.01 . . . . . 26520 1 7 . 1 1 10 10 THR N N 15 1.45 0.01 . . . . . 26520 1 8 . 1 1 11 11 TRP N N 15 1.41 0.01 . . . . . 26520 1 9 . 1 1 12 12 ASN N N 15 1.37 0.01 . . . . . 26520 1 10 . 1 1 13 13 THR N N 15 1.23 0.01 . . . . . 26520 1 11 . 1 1 14 14 LYS N N 15 1.45 0.01 . . . . . 26520 1 12 . 1 1 15 15 GLU N N 15 1.39 0.01 . . . . . 26520 1 13 . 1 1 16 16 GLU N N 15 1.44 0.01 . . . . . 26520 1 14 . 1 1 17 17 ALA N N 15 1.47 0.01 . . . . . 26520 1 15 . 1 1 18 18 LYS N N 15 1.40 0.01 . . . . . 26520 1 16 . 1 1 19 19 GLN N N 15 1.41 0.01 . . . . . 26520 1 17 . 1 1 20 20 ALA N N 15 1.48 0.01 . . . . . 26520 1 18 . 1 1 21 21 PHE N N 15 1.52 0.01 . . . . . 26520 1 19 . 1 1 22 22 LYS N N 15 1.42 0.01 . . . . . 26520 1 20 . 1 1 23 23 GLU N N 15 1.44 0.01 . . . . . 26520 1 21 . 1 1 24 24 LEU N N 15 1.47 0.01 . . . . . 26520 1 22 . 1 1 25 25 LEU N N 15 1.46 0.01 . . . . . 26520 1 23 . 1 1 26 26 LYS N N 15 1.42 0.01 . . . . . 26520 1 24 . 1 1 27 27 GLU N N 15 1.43 0.01 . . . . . 26520 1 25 . 1 1 28 28 LYS N N 15 1.44 0.01 . . . . . 26520 1 26 . 1 1 29 29 ARG N N 15 1.41 0.01 . . . . . 26520 1 27 . 1 1 30 30 VAL N N 15 1.42 0.01 . . . . . 26520 1 28 . 1 1 32 32 SER N N 15 1.38 0.01 . . . . . 26520 1 29 . 1 1 33 33 ASN N N 15 1.48 0.01 . . . . . 26520 1 30 . 1 1 34 34 ALA N N 15 1.39 0.01 . . . . . 26520 1 31 . 1 1 35 35 SER N N 15 1.46 0.01 . . . . . 26520 1 32 . 1 1 36 36 TRP N N 15 1.43 0.01 . . . . . 26520 1 33 . 1 1 37 37 GLU N N 15 1.38 0.01 . . . . . 26520 1 34 . 1 1 38 38 GLN N N 15 1.41 0.01 . . . . . 26520 1 35 . 1 1 39 39 ALA N N 15 1.43 0.01 . . . . . 26520 1 36 . 1 1 40 40 MET N N 15 1.42 0.01 . . . . . 26520 1 37 . 1 1 41 41 LYS N N 15 1.36 0.01 . . . . . 26520 1 38 . 1 1 42 42 MET N N 15 1.41 0.01 . . . . . 26520 1 39 . 1 1 43 43 ILE N N 15 1.41 0.01 . . . . . 26520 1 40 . 1 1 44 44 ILE N N 15 1.38 0.01 . . . . . 26520 1 41 . 1 1 45 45 ASN N N 15 1.36 0.01 . . . . . 26520 1 42 . 1 1 46 46 ASP N N 15 1.17 0.01 . . . . . 26520 1 43 . 1 1 48 48 ARG N N 15 1.46 0.01 . . . . . 26520 1 44 . 1 1 50 50 SER N N 15 1.44 0.01 . . . . . 26520 1 45 . 1 1 51 51 ALA N N 15 1.48 0.01 . . . . . 26520 1 46 . 1 1 52 52 LEU N N 15 1.41 0.01 . . . . . 26520 1 47 . 1 1 53 53 ALA N N 15 1.33 0.01 . . . . . 26520 1 48 . 1 1 54 54 LYS N N 15 1.32 0.01 . . . . . 26520 1 49 . 1 1 55 55 LEU N N 15 1.45 0.01 . . . . . 26520 1 50 . 1 1 56 56 SER N N 15 1.41 0.01 . . . . . 26520 1 51 . 1 1 57 57 GLU N N 15 1.39 0.01 . . . . . 26520 1 52 . 1 1 58 58 LYS N N 15 1.47 0.01 . . . . . 26520 1 53 . 1 1 59 59 LYS N N 15 1.43 0.01 . . . . . 26520 1 54 . 1 1 60 60 GLN N N 15 1.38 0.01 . . . . . 26520 1 55 . 1 1 61 61 ALA N N 15 1.44 0.01 . . . . . 26520 1 56 . 1 1 62 62 PHE N N 15 1.47 0.01 . . . . . 26520 1 57 . 1 1 63 63 ASN N N 15 1.43 0.01 . . . . . 26520 1 58 . 1 1 64 64 ALA N N 15 1.41 0.01 . . . . . 26520 1 59 . 1 1 65 65 TYR N N 15 1.43 0.01 . . . . . 26520 1 60 . 1 1 66 66 LYS N N 15 1.45 0.01 . . . . . 26520 1 61 . 1 1 67 67 VAL N N 15 1.39 0.01 . . . . . 26520 1 62 . 1 1 68 68 GLN N N 15 1.46 0.01 . . . . . 26520 1 63 . 1 1 69 69 THR N N 15 1.56 0.01 . . . . . 26520 1 64 . 1 1 70 70 GLU N N 15 1.54 0.01 . . . . . 26520 1 65 . 1 1 71 71 LYS N N 15 1.50 0.01 . . . . . 26520 1 stop_ save_