save_chem_shift_perturbation_1 _Chem_shift_perturbation_list.Sf_category chem_shift_perturbation _Chem_shift_perturbation_list.Sf_framecode chem_shift_perturbation_1 _Chem_shift_perturbation_list.Entry_ID 50449 _Chem_shift_perturbation_list.ID 1 _Chem_shift_perturbation_list.Name 'XcpH Chemical shift perturbation' _Chem_shift_perturbation_list.Type 'macromolecular binding' _Chem_shift_perturbation_list.Entity_assembly_ID . _Chem_shift_perturbation_list.Titrated_entity_assembly_name . _Chem_shift_perturbation_list.Sample_condition_list_ID 2 _Chem_shift_perturbation_list.Sample_condition_list_label $sample_conditions_2 _Chem_shift_perturbation_list.Chem_shift_ref_set_ID 1 _Chem_shift_perturbation_list.Chem_shift_ref_set_label $chem_shift_reference_1 _Chem_shift_perturbation_list.Details ; XcpH was mixed with increasing concentrations of XcpJ soluble domain and a 2D 1H-15N HSQC spectrum was collected for each sample. Chemical shift perturbation is reported for the change in signal from the sample without XcpJ and the sample with most XcpJ (80 uM). ; _Chem_shift_perturbation_list.Text_data_format . _Chem_shift_perturbation_list.Text_data . loop_ _Chem_shift_perturbation_experiment.Experiment_ID _Chem_shift_perturbation_experiment.Experiment_name _Chem_shift_perturbation_experiment.Sample_ID _Chem_shift_perturbation_experiment.Sample_label _Chem_shift_perturbation_experiment.Sample_state _Chem_shift_perturbation_experiment.Entry_ID _Chem_shift_perturbation_experiment.Chem_shift_perturbation_list_ID 1 '2D 1H-15N HSQC' . . . 50449 1 15 '2D 1H-15N HSQC' . . . 50449 1 16 '2D 1H-15N HSQC' . . . 50449 1 17 '2D 1H-15N HSQC' . . . 50449 1 stop_ loop_ _Chem_shift_perturbation_software.Software_ID _Chem_shift_perturbation_software.Software_label _Chem_shift_perturbation_software.Method_ID _Chem_shift_perturbation_software.Method_label _Chem_shift_perturbation_software.Entry_ID _Chem_shift_perturbation_software.Chem_shift_perturbation_list_ID 3 $software_3 . . 50449 1 stop_ loop_ _Chem_shift_perturbation.ID _Chem_shift_perturbation.Assembly_atom_ID _Chem_shift_perturbation.Entity_assembly_ID _Chem_shift_perturbation.Entity_ID _Chem_shift_perturbation.Comp_index_ID _Chem_shift_perturbation.Seq_ID _Chem_shift_perturbation.Comp_ID _Chem_shift_perturbation.Atom_ID _Chem_shift_perturbation.Atom_type _Chem_shift_perturbation.Atom_isotope_number _Chem_shift_perturbation.Titration_value _Chem_shift_perturbation.Titration_value_err _Chem_shift_perturbation.Chem_shift_val _Chem_shift_perturbation.Chem_shift_val_err _Chem_shift_perturbation.Difference_chem_shift_val _Chem_shift_perturbation.Difference_chem_shift_val_err _Chem_shift_perturbation.Resonance_ID _Chem_shift_perturbation.Auth_entity_assembly_ID _Chem_shift_perturbation.Auth_seq_ID _Chem_shift_perturbation.Auth_comp_ID _Chem_shift_perturbation.Auth_atom_ID _Chem_shift_perturbation.Entry_ID _Chem_shift_perturbation.Chem_shift_perturbation_list_ID 1 . 1 1 8 8 THR H H 1 . . . . 0.036636412 . . . 29 THR . 50449 1 2 . 1 1 12 12 LEU H H 1 . . . . 0.081231624 . . . 33 LEU . 50449 1 3 . 1 1 13 13 ASP H H 1 . . . . 0.013798188 . . . 34 ASP . 50449 1 4 . 1 1 14 14 SER H H 1 . . . . 0.047878248 . . . 35 SER . 50449 1 5 . 1 1 16 16 ALA H H 1 . . . . 0.027656645 . . . 37 ALA . 50449 1 6 . 1 1 19 19 LEU H H 1 . . . . 0.049228616 . . . 40 LEU . 50449 1 7 . 1 1 20 20 ALA H H 1 . . . . 0.080941378 . . . 41 ALA . 50449 1 8 . 1 1 21 21 GLY H H 1 . . . . 0.174553258 . . . 42 GLY . 50449 1 9 . 1 1 25 25 VAL H H 1 . . . . 0.025595052 . . . 46 VAL . 50449 1 10 . 1 1 27 27 THR H H 1 . . . . 0.086057403 . . . 48 THR . 50449 1 11 . 1 1 30 30 ALA H H 1 . . . . 0.114406323 . . . 51 ALA . 50449 1 12 . 1 1 34 34 ASN H H 1 . . . . 0.111257839 . . . 55 ASN . 50449 1 13 . 1 1 38 38 GLY H H 1 . . . . 0.108606891 . . . 59 GLY . 50449 1 14 . 1 1 39 39 LEU H H 1 . . . . 0.036584377 . . . 60 LEU . 50449 1 15 . 1 1 40 40 ARG H H 1 . . . . 0.014713599 . . . 61 ARG . 50449 1 16 . 1 1 42 42 GLU H H 1 . . . . 0.032733876 . . . 63 GLU . 50449 1 17 . 1 1 43 43 ARG H H 1 . . . . 0.087279723 . . . 64 ARG . 50449 1 18 . 1 1 44 44 ASP H H 1 . . . . 0.020821623 . . . 65 ASP . 50449 1 19 . 1 1 45 45 ALA H H 1 . . . . 0.018760064 . . . 66 ALA . 50449 1 20 . 1 1 46 46 TYR H H 1 . . . . 0.065534393 . . . 67 TYR . 50449 1 21 . 1 1 47 47 GLN H H 1 . . . . 0.035378336 . . . 68 GLN . 50449 1 22 . 1 1 48 48 VAL H H 1 . . . . 0.03039013 . . . 69 VAL . 50449 1 23 . 1 1 49 49 LEU H H 1 . . . . 0.048709616 . . . 70 LEU . 50449 1 24 . 1 1 50 50 ARG H H 1 . . . . 0.033532472 . . . 71 ARG . 50449 1 25 . 1 1 51 51 TYR H H 1 . . . . 0.047374325 . . . 72 TYR . 50449 1 26 . 1 1 53 53 GLU H H 1 . . . . 0.039817793 . . . 74 GLU . 50449 1 27 . 1 1 54 54 ALA H H 1 . . . . 0.047748054 . . . 75 ALA . 50449 1 28 . 1 1 56 56 ALA H H 1 . . . . 0.039317384 . . . 77 ALA . 50449 1 29 . 1 1 57 57 ARG H H 1 . . . . 0.091014871 . . . 78 ARG . 50449 1 30 . 1 1 58 58 TRP H H 1 . . . . 0.040232657 . . . 79 TRP . 50449 1 31 . 1 1 59 59 LEU H H 1 . . . . 0.034004804 . . . 80 LEU . 50449 1 32 . 1 1 65 65 SER H H 1 . . . . 0.036816527 . . . 86 SER . 50449 1 33 . 1 1 67 67 ARG H H 1 . . . . 0.029466195 . . . 88 ARG . 50449 1 34 . 1 1 68 68 LEU H H 1 . . . . 0.033903933 . . . 89 LEU . 50449 1 35 . 1 1 70 70 GLU H H 1 . . . . 0.015010885 . . . 91 GLU . 50449 1 36 . 1 1 71 71 TRP H H 1 . . . . 0.036842593 . . . 92 TRP . 50449 1 37 . 1 1 72 72 ALA H H 1 . . . . 0.036365047 . . . 93 ALA . 50449 1 38 . 1 1 73 73 GLU H H 1 . . . . 0.054341574 . . . 94 GLU . 50449 1 39 . 1 1 74 74 LEU H H 1 . . . . 0.027178607 . . . 95 LEU . 50449 1 40 . 1 1 75 75 THR H H 1 . . . . 0.114252381 . . . 96 THR . 50449 1 41 . 1 1 80 80 GLY H H 1 . . . . 0.121979821 . . . 101 GLY . 50449 1 42 . 1 1 85 85 LEU H H 1 . . . . 0.035444699 . . . 106 LEU . 50449 1 43 . 1 1 86 86 ALA H H 1 . . . . 0.101698083 . . . 107 ALA . 50449 1 44 . 1 1 87 87 GLY H H 1 . . . . 0.061809519 . . . 108 GLY . 50449 1 45 . 1 1 97 97 GLY H H 1 . . . . 0.03070304 . . . 118 GLY . 50449 1 46 . 1 1 98 98 THR H H 1 . . . . 0.039374357 . . . 119 THR . 50449 1 47 . 1 1 99 99 ASP H H 1 . . . . 0.128192733 . . . 120 ASP . 50449 1 48 . 1 1 100 100 GLN H H 1 . . . . 0.03773429 . . . 121 GLN . 50449 1 49 . 1 1 102 102 GLN H H 1 . . . . 0.055634582 . . . 123 GLN . 50449 1 50 . 1 1 103 103 LEU H H 1 . . . . 0.072915476 . . . 124 LEU . 50449 1 51 . 1 1 104 104 LEU H H 1 . . . . 0.138517424 . . . 125 LEU . 50449 1 52 . 1 1 105 105 ILE H H 1 . . . . 0.035888578 . . . 126 ILE . 50449 1 53 . 1 1 109 109 GLY H H 1 . . . . 0.188240821 . . . 130 GLY . 50449 1 54 . 1 1 110 110 GLU H H 1 . . . . 0.132274626 . . . 131 GLU . 50449 1 55 . 1 1 114 114 PHE H H 1 . . . . 0.18845744 . . . 135 PHE . 50449 1 56 . 1 1 115 115 ARG H H 1 . . . . 0.098339005 . . . 136 ARG . 50449 1 57 . 1 1 116 116 LEU H H 1 . . . . 0.323437768 . . . 137 LEU . 50449 1 58 . 1 1 117 117 ARG H H 1 . . . . 0.160339255 . . . 138 ARG . 50449 1 59 . 1 1 118 118 LEU H H 1 . . . . 0.095397205 . . . 139 LEU . 50449 1 60 . 1 1 119 119 ALA H H 1 . . . . 0.019627532 . . . 140 ALA . 50449 1 61 . 1 1 120 120 GLU H H 1 . . . . 0.03569351 . . . 141 GLU . 50449 1 62 . 1 1 121 121 ARG H H 1 . . . . 0.022873784 . . . 142 ARG . 50449 1 63 . 1 1 122 122 GLY H H 1 . . . . 0.02586033 . . . 143 GLY . 50449 1 64 . 1 1 124 124 GLU H H 1 . . . . 0.022776303 . . . 145 GLU . 50449 1 65 . 1 1 125 125 GLY H H 1 . . . . 0.033380783 . . . 146 GLY . 50449 1 66 . 1 1 126 126 ARG H H 1 . . . . 0.048909781 . . . 147 ARG . 50449 1 67 . 1 1 128 128 LEU H H 1 . . . . 0.07997037 . . . 149 LEU . 50449 1 68 . 1 1 132 132 SER H H 1 . . . . 0.173563869 . . . 153 SER . 50449 1 69 . 1 1 141 141 GLU H H 1 . . . . 0.292943043 . . . 162 GLU . 50449 1 70 . 1 1 143 143 ALA H H 1 . . . . 0.195994107 . . . 164 ALA . 50449 1 71 . 1 1 145 145 ARG H H 1 . . . . 0.027527259 . . . 166 ARG . 50449 1 stop_ save_